ABSTRACT
BACKGROUND: Platelet-rich plasma (PRP) treatment is a promising tool for dermal tissue regeneration. PRP combined with subcision can synergistically induce dermal tissue regeneration. OBJECTIVE: The purpose of this study was to investigate the effects of PRP on the proliferation and migration of skin fibroblasts, as well as on the type I collagen, matrix metalloproteinase (MMP)-1, and MMP-2 expression in these skin cells. The effect of PRP with subcision on the expression of TGF-beta1 was also investigated in an animal model. METHODS: Human skin fibroblasts were treated with various concentrations of PRP. The proliferation and migration rate of the cells were evaluated by the trypan blue exclusion method and scratch assay, respectively. The expression levels of type I collagen, MMP-1, and MMP-2 were analyzed by western blot or RT-PCR. In addition, the activity levels of MMP-1 and MMP-2 were studied by zymography. Finally, we treated the animal back with PRP, subcision, or PRP with subcision. The specimens were evaluated by H&E, Masson-trichrome, and TGF-beta1 immunohistochemical staining. RESULTS: Data from this study showed that PRP more effectively promoted the migration and proliferation of cells in a dose-dependent manner. The expression levels of type I collagen, MMP-1, and MMP-2 were increased in PRP-treated fibroblasts at the protein and mRNA levels. The in vivo study revealed that the expression of TGF-beta1 was prominently increased by co-treatment with PRP and subcision rather than by treatment with either PRP or subcision alone. CONCLUSION: PRP treatment promoted fibroblast migration and proliferation, and increased the expression of type I collagen, MMP-1, MMP-2, and TGF-beta1. Therefore, PRP co-application with subcision is an effective method for dermal remodeling and can be a good treatment option for depressed acne scars.
Subject(s)
Animals , Humans , Acne Vulgaris , Blotting, Western , Cicatrix , Collagen Type I , Fibroblasts , Models, Animal , Platelet-Rich Plasma , Regeneration , RNA, Messenger , Skin , Transforming Growth Factor beta1 , Trypan BlueABSTRACT
BACKGROUND: Platelet-rich plasma (PRP) treatment is a promising tool for dermal tissue regeneration. PRP combined with subcision can synergistically induce dermal tissue regeneration. OBJECTIVE: The purpose of this study was to investigate the effects of PRP on the proliferation and migration of skin fibroblasts, as well as on the type I collagen, matrix metalloproteinase (MMP)-1, and MMP-2 expression in these skin cells. The effect of PRP with subcision on the expression of TGF-beta1 was also investigated in an animal model. METHODS: Human skin fibroblasts were treated with various concentrations of PRP. The proliferation and migration rate of the cells were evaluated by the trypan blue exclusion method and scratch assay, respectively. The expression levels of type I collagen, MMP-1, and MMP-2 were analyzed by western blot or RT-PCR. In addition, the activity levels of MMP-1 and MMP-2 were studied by zymography. Finally, we treated the animal back with PRP, subcision, or PRP with subcision. The specimens were evaluated by H&E, Masson-trichrome, and TGF-beta1 immunohistochemical staining. RESULTS: Data from this study showed that PRP more effectively promoted the migration and proliferation of cells in a dose-dependent manner. The expression levels of type I collagen, MMP-1, and MMP-2 were increased in PRP-treated fibroblasts at the protein and mRNA levels. The in vivo study revealed that the expression of TGF-beta1 was prominently increased by co-treatment with PRP and subcision rather than by treatment with either PRP or subcision alone. CONCLUSION: PRP treatment promoted fibroblast migration and proliferation, and increased the expression of type I collagen, MMP-1, MMP-2, and TGF-beta1. Therefore, PRP co-application with subcision is an effective method for dermal remodeling and can be a good treatment option for depressed acne scars.
Subject(s)
Animals , Humans , Acne Vulgaris , Blotting, Western , Cicatrix , Collagen Type I , Fibroblasts , Models, Animal , Platelet-Rich Plasma , Regeneration , RNA, Messenger , Skin , Transforming Growth Factor beta1 , Trypan BlueABSTRACT
Various methods have been used to correct the prominent ear, in which the deformity is due to lack of formation of the normal antihelix. In 1963, Mustard presented a method which creates the antihelical fold by placing permanent mattress sutures through the cartilage without using any actual cartilaginous incisions. However, the main disadvantage of this method is difficulty in accurate full- thickness transcartilaginous suture without an injury to the anterior auricular skin. To solve this problem, the authors separated the anterior auricular skin from the cartilage by a subcision method using a 21-gauge needle without making a skin incision. Between 2001 and 2002, a total of 16 prominent ears in 12 patients were corrected by this method. Eight patients underwent unilateral correction and four patients underwent bilateral correction. Seven were female and 5 were male. Patients' age at operation ranged from 5 to 24 years(mean 9.7 years). Patients were followed up from 5 to 14 months(mean 8.6 months). No major complications were observed while using this method and good aesthetic result was achieved in all cases, except one recurrence case that needed revision procedure. This method is a simple and safe technique with reliable aesthetic results and no scar on the anterior auricular skin.